Human TIMP2 Recombinant Protein (RPPB2325)

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SKU:
RPPB2325
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
TIMP2
Synonyms:
Fibroblast growth factor 17
Synonyms:
FGF-17
Synonyms:
FGF17
Synonyms:
FGF-13
Source:
Escherichia Coli
Uniprot:
P16035
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Description

Product Name:Human TIMP2 Recombinant Protein
Product Code:RPPB2325
Size:20µg
Species:Human
Target:TIMP2
Synonyms:Fibroblast growth factor 17, FGF-17, FGF17, FGF-13, HH20.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:TIMP2 protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 3 % trehalose.
Solubility:It is recommended to reconstitute the lyophilized TIMP2 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized TIMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIMP2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by: (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG AAPPKQEFLD IEDP
Biological Activity:Fully biologically active when compared to standard. The biologically active as determined by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.

TIMP2 belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases that take part in degradation of the extracellular matrix. Besides having an inhibitory role against metalloproteinases, the encoded protein has a exclusive part among TIMP family members in its capability to directly suppress the proliferation of endothelial cells. Consequently, the encoded protein is crucial to the conservation of tissue homeostasis by suppressing the production of quiescent tissues as an answer to angiogenic factors, and by inhibiting protease activity in tissues undergoing renovation of the extracellular matrix.

TIMP2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 194 amino acids and having a molecular mass of 21.8kDa. 

UniProt Protein Function:TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis; Cell development/differentiation; Inhibitor

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: extracellular space; proteinaceous extracellular matrix; growth cone; cell surface; cell soma; extracellular region; basement membrane

Molecular Function:integrin binding; metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding; enzyme activator activity

Biological Process: response to drug; regulation of Rap protein signal transduction; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; central nervous system development; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging

NCBI Summary:This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]
UniProt Code:P16035
NCBI GenInfo Identifier:135854
NCBI Gene ID:7077
NCBI Accession:P16035.2
UniProt Secondary Accession:P16035,Q16121, Q93006, Q9UDF7,
UniProt Related Accession:P16035,Q96MC4
Molecular Weight:24,399 Da
NCBI Full Name:Metalloproteinase inhibitor 2
NCBI Synonym Full Names:TIMP metallopeptidase inhibitor 2
NCBI Official Symbol:TIMP2  
NCBI Official Synonym Symbols:DDC8; CSC-21K  
NCBI Protein Information:metalloproteinase inhibitor 2; TIMP-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2
UniProt Protein Name:Metalloproteinase inhibitor 2
UniProt Synonym Protein Names:CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2
Protein Family:Metalloproteinase inhibitor
UniProt Gene Name:TIMP2  
UniProt Entry Name:TIMP2_HUMAN
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