Human RNASE3 Recombinant Protein (RPPB2247)

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SKU:
RPPB2247
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
RNASE3
Synonyms:
ECP
Synonyms:
RNS3
Synonyms:
Ribonuclease 3
Synonyms:
Eosinophil cationic protein
Source:
Sf9 Insect cells
Uniprot:
P12724
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Description

Product Name:Human RNASE3 Recombinant Protein
Product Code:RPPB2247
Size:10µg
Species:Human
Target:RNASE3
Synonyms:ECP, RNS3, Ribonuclease 3, Eosinophil cationic protein, RNASE3, RNASE3.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:RNASE3 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:ADPRPPQFTR AQWFAIQHIS LNPPRCTIAM RAINNYRWRC KNQNTFLRTT FANVVNVCGN QSIRCPHNRT LNNCHRSRFR VPLLHCDLIN PGAQNISNCR YADRPGRRFY VVACDNRDPR DSPRYPVVPV HLDTTIHHHH HH

Ribonuclease 3 (RNASE3) is cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. RNASE3 possesses a broad diversity of biological activities. RNASE3 protein has shown antibacterial activity such as cytoplasmic membrane depolarization of preferentially Gram-negative and Gram-positive strains and promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

RNASE3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 142 amino acids (28-160 a.a.) and having a molecular mass of 16.6kDa (Migrates at 18-28kDa on SDS-PAGE under reducing conditions).RNASE3 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:RNASE3: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Belongs to the pancreatic ribonuclease family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; EC 3.1.27.-; Ribonuclease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space; extracellular region

Molecular Function:nucleic acid binding; endonuclease activity; ribonuclease activity

Biological Process: defense response to Gram-positive bacterium; innate immune response in mucosa; RNA catabolic process; antibacterial humoral response

NCBI Summary:The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]
UniProt Code:P12724
NCBI GenInfo Identifier:147744558
NCBI Gene ID:6037
NCBI Accession:P12724.2
UniProt Secondary Accession:P12724,Q4VBC1, Q8WTP7, Q8WZ62, Q9GZN9,
UniProt Related Accession:P12724
Molecular Weight:18kDa
NCBI Full Name:Eosinophil cationic protein
NCBI Synonym Full Names:ribonuclease, RNase A family, 3
NCBI Official Symbol:RNASE3  
NCBI Official Synonym Symbols:ECP; RNS3  
NCBI Protein Information:eosinophil cationic protein; RNase 3; ribonuclease 3; cytotoxic ribonuclease
UniProt Protein Name:Eosinophil cationic protein
UniProt Synonym Protein Names:Ribonuclease 3; RNase 3
Protein Family:Eosinophil cationic protein
UniProt Gene Name:RNASE3  
UniProt Entry Name:ECP_HUMAN
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