Human PURB Recombinant Protein (RPPB4338)

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SKU:
RPPB4338
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
PURB
Synonyms:
Transcriptional activator protein Pur-beta
Synonyms:
Purine-rich element-binding protein B
Synonyms:
PURB
Synonyms:
PURBETA
Source:
Escherichia Coli
Uniprot:
Q96QR8
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Description

Product Name:Human PURB Recombinant Protein
Product Code:RPPB4338
Size:20µg
Species:Human
Target:PURB
Synonyms:Transcriptional activator protein Pur-beta, Purine-rich element-binding protein B, PURB, PURBETA.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PURB solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMADGDSG SERGGGGGPC GFQPASRGGG EQETQELASK RLDIQNKRFY LDVKQNAKGR FLKIAEVGAG GSKSRLTLSM AVAAEFRDSL GDFIEHYAQL GPSSPEQLAA GAEEGGGPRR ALKSEFLVRE NRKYYLDLKE NQRGRFLRIR QTVNRGGGGF GAGPGPGGLQ SGQTIALPAQ GLIEFRDALA KLIDDYGGED DELAGGPGGG AGGPGGGLYG ELPEGTSITV DSKRFFFDVG CNKYGVFLRV SEVKPSYRNA ITVPFKAWGK FGGAFCRYAD EMKEIQERQR DKLYERRGGG SGGGEESEGE EVDED

Purine-Rich Element Binding Protein B (PURB) is a single-stranded DNA-binding protein which takes part in the dendritic transport of a subset of mRNAs. PURB operates as repressor in myoblasts and fibroblasts in the control of vascular smooth muscle alpha-actin gene transcription. PURB binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in various eukaryotes from yeasts through humans.

PURB Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 335 amino acids (1-312) and having a molecular mass of 35.6 kDa.PURB is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:Pur-beta: Has capacity to bind repeated elements in single- stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine- rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs. Belongs to the PUR DNA-binding protein family.
UniProt Protein Details:

Protein type:RNA-binding; DNA-binding

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: DNA replication factor A complex; nucleus

Molecular Function:mRNA binding; single-stranded DNA binding; transcription factor binding

NCBI Summary:This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]
UniProt Code:Q96QR8
NCBI GenInfo Identifier:74732688
NCBI Gene ID:5814
NCBI Accession:Q96QR8.3
UniProt Secondary Accession:Q96QR8,A4D2L7,
UniProt Related Accession:Q96QR8
Molecular Weight:33,241 Da
NCBI Full Name:Transcriptional activator protein Pur-beta
NCBI Synonym Full Names:purine rich element binding protein B
NCBI Official Symbol:PURB  
NCBI Official Synonym Symbols:PURBETA  
NCBI Protein Information:transcriptional activator protein Pur-beta
UniProt Protein Name:Transcriptional activator protein Pur-beta
UniProt Synonym Protein Names:Purine-rich element-binding protein B
Protein Family:Transcriptional activator protein
UniProt Gene Name:PURB  
UniProt Entry Name:PURB_HUMAN
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