Human PSMB9 Recombinant Protein (RPPB2212)

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SKU:
RPPB2212
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
PSMB9
Synonyms:
Proteasome subunit beta type-9
Synonyms:
Low molecular mass protein 2
Synonyms:
Macropain chain 7
Synonyms:
Multicatalytic endopeptidase complex chain 7
Source:
Escherichia Coli
Uniprot:
P28065
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Description

Product Name:Human PSMB9 Recombinant Protein
Product Code:RPPB2212
Size:10µg
Species:Human
Target:PSMB9
Synonyms:Proteasome subunit beta type-9, Low molecular mass protein 2, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Proteasome chain 7, Proteasome subunit beta-1i, Really interesting new gene 12 protein, PSMB9, LMP2, PSMB6i, RING12, beta1i, MGC70470.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:PSMB9 solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 50mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MTTIMAVEFD GGVVMGSDSR VSAGEAVVNR VFDKLSPLHE RIYCALSGS AADAQAVADMA AYQLELHGIE LEEPPLVLAA ANVVRNISYK YREDLSAHLM VAGWDQREGG QVYGTLGGML TRQPFAIGGS GSTFIYGYVD AAYKPGMSPE ECRRFTTDAI ALAMSRDGSS GGVIYLVTIT AAGVDHRVIL GNELPKFYDE

Proteasome subunit beta type-9 (PSMB9) belongs to the proteasome B-type family which is a 20S core beta subunit. PSMB9 is positioned in the class II region of the MHC (major histocompatibility complex). Expression of the PSMB9 protein is induced by INFG and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome.

PSMB9 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 220 amino acids (21-219 a.a.) and having a molecular mass of 23.5kDa.PSMB9 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PSMB9: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.4.25.1; Nuclear receptor co-regulator; Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytoplasm; cytosol

Molecular Function:threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle

NCBI Summary:The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar 2010]
UniProt Code:P28065
NCBI GenInfo Identifier:417529
NCBI Gene ID:5698
NCBI Accession:P28065.2
UniProt Secondary Accession:P28065,P28076,
UniProt Related Accession:P28065
Molecular Weight:
NCBI Full Name:Proteasome subunit beta type-9
NCBI Synonym Full Names:proteasome 20S subunit beta 9
NCBI Official Symbol:PSMB9  
NCBI Official Synonym Symbols:LMP2; PRAAS3; PSMB6i; RING12; beta1i  
NCBI Protein Information:proteasome subunit beta type-9
UniProt Protein Name:Proteasome subunit beta type-9
UniProt Synonym Protein Names:Low molecular mass protein 2; Macropain chain 7; Multicatalytic endopeptidase complex chain 7; Proteasome chain 7; Proteasome subunit beta-1i; Really interesting new gene 12 protein
Protein Family:Latent membrane protein
UniProt Gene Name:PSMB9  
UniProt Entry Name:PSB9_HUMAN
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