The Human Pregnancy Zone Protein (PZP) ELISA Kit is specifically designed for the precise measurement of PZP levels in human serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring consistent and accurate results for various research purposes. PZP, also known as Pregnancy Zone Protein, is an important protein involved in pregnancy and fetal development. It plays a critical role in maintaining pregnancy by modulating the immune response and promoting proper fetal growth. Studies have shown that abnormalities in PZP levels are associated with pregnancy complications, making it a valuable biomarker for monitoring pregnancy health.
Whether studying reproductive health, fetal development, or pregnancy-related disorders, the Human PZP ELISA Kit offers a reliable tool for researchers to investigate the role of PZP and its implications in various clinical conditions. With its high-quality components and standardized protocols, this kit ensures accurate and reproducible results, facilitating meaningful discoveries in the field of pregnancy research.
Product Name:
Human Pregnancy Zone Protein/PZP ELISA Kit
SKU:
HUFI01034
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ug/mL
Range:
0.156-10 ug/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-104%
89-105%
86-99%
EDTA Plasma (n = 5)
82-90%
87-100%
84-100%
Heparin Plasma (n = 5)
89-99%
85-100%
84-97%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-105
98
EDTA Plasma (n = 5)
89-102
97
Heparin Plasma (n = 5)
89-103
98
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
