Human PRDM15 (PR domain zinc finger protein 15) ELISA Kit
The Human PRDM15 (PR Domain Zinc Finger Protein 15) ELISA Kit is a premium assay designed for the precise quantification of PRDM15 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and reliable results, making it an indispensable tool for various research studies. PRDM15 is a key regulator in gene expression and cell differentiation, playing a crucial role in developmental processes and stem cell maintenance.
Dysregulation of PRDM15 has been linked to various diseases such as cancer, developmental disorders, and neurological conditions, highlighting its importance as a potential therapeutic target and biomarker for disease progression. With the Human PRDM15 ELISA Kit, researchers can delve deeper into the functions and mechanisms of PRDM15, paving the way for a better understanding of its role in health and disease. Order your kit now and unlock the potential of PRDM15 research.
Product Name:
Human PRDM15 (PR domain zinc finger protein 15) ELISA Kit
SKU:
HUFI08594
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-103%
82-101%
82-98%
EDTA Plasma (n = 5)
86-105%
84-101%
81-93%
Heparin Plasma (n = 5)
87-102%
84-99%
83-96%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-99
91
EDTA Plasma (n = 5)
87-103
97
Heparin Plasma (n = 5)
91-105
99
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
