Human PPP1CA Recombinant Protein (RPPB2162)

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SKU:
RPPB2162
Product type:
Recombinant Protein
Size:
5ug
Species:
Human
Target:
PPP1CA
Synonyms:
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Synonyms:
PP-1A
Synonyms:
PPP1CA
Synonyms:
PPP1A
Source:
Escherichia Coli
Uniprot:
P62136
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Description

Product Name:Human PPP1CA Recombinant Protein
Product Code:RPPB2162
Size:5µg
Species:Human
Target:PPP1CA
Synonyms:Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A, PP1alpha.
Source:Escherichia Coli
Physical Appearance:Sterile filtered yellowish solution.
Formulation:PPP1CA protein solution (0.2mg/ml) containing 50mM Tris-HCl (pH8.5),0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2 and 50% glycerol. 
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK
Biological Activity:Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 30mM of substrate.

Serine/Threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) is crucial for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. The PPP1CA protein may have a significant role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

PPP1CA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 350 amino acids (1-330 a.a) and having a molecular mass of 39.7kDa.PPP1CA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PPP1CA: is a catalytic subunit of protein phosphatase 1 (PP1), aubiquitous cytosolic serine-threonine phosphatase. Composed of a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer's disease. Down-regulated in lung squamous cell carcinoma.
UniProt Protein Details:

Protein type:EC 3.1.3.16; Nucleolus; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; nuclear chromosome, telomeric region; nucleoplasm; nucleus; plasma membrane

Molecular Function:phosphoprotein phosphatase activity; phosphoric monoester hydrolase activity; protein binding

Biological Process: circadian regulation of gene expression; dephosphorylation; entrainment of circadian clock by photoperiod; negative regulation of protein binding; protein amino acid dephosphorylation; regulation of circadian rhythm

NCBI Summary:The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P62136
NCBI GenInfo Identifier:49065811
NCBI Gene ID:5499
NCBI Accession:P62136.1
UniProt Secondary Accession:P62136,P08129, P20653, P22802, Q07161, A6NNR3, B2R908
UniProt Related Accession:P62136
Molecular Weight:Calculated MW: 32kDa/37kDa/38kDaObserved MW: 38kDa
NCBI Full Name:Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
NCBI Synonym Full Names:protein phosphatase 1 catalytic subunit alpha
NCBI Official Symbol:PPP1CA  
NCBI Official Synonym Symbols:PP1A; PP-1A; PPP1A; PP1alpha  
NCBI Protein Information:serine/threonine-protein phosphatase PP1-alpha catalytic subunit
UniProt Protein Name:Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Protein Family:Serine/threonine-protein phosphatase
UniProt Gene Name:PPP1CA  
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