Human LCAT Recombinant Protein (RPPB1884)

(No reviews yet) Write a Review

제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.

주문
SKU:
RPPB1884
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
LCAT
Synonyms:
Phosphatidylcholine-sterol acyltransferase
Synonyms:
Lecithin-cholesterol acyltransferase
Synonyms:
Phospholipid-cholesterol acyltransferase
Synonyms:
LCAT
Source:
Escherichia Coli
Uniprot:
P04180
€0.00
Frequently bought together:

Description

Product Name:Human LCAT Recombinant Protein
Product Code:RPPB1884
Size:20µg
Species:Human
Target:LCAT
Synonyms:Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase, LCAT.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:LCAT protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMFWLLN VLFPPHTTPK AELSNHTRPV ILVPGCLGNQ LEAKLDKPDV VNWMCYRKTE DFFTIWLDLN MFLPLGVDCW IDNTRVVYNR SSGLVSNAPG VQIRVPGFGK TYSVEYLDSS KLAGYLHTLV QNLVNNGYVR DETVRAAPYD WRLEPGQQEE YYRKLAGLVE EMHAAYGKPV FLIGHSLGCL HLLYFLLRQP QAWKDRFIDG FISLGAPWGG SIKPMLVLAS GDNQGIPIMS SIKLKEEQRI TTTSPWMFPS RMAWPEDHVF ISTPSFNYTG RDFQRFFADL HFEEGWYMWL QSRDLLAGLP APGVEVYCLY GVGLPTPRTY IYDHGFPYTD PVGVLYEDGD DTVATRSTEL CGLWQGRQPQ PVHLLPLHGI QHLNMVFSNL TLEHINAILL GAYRQGPPAS PTASPEPPPP E

LCAT is an extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. LCAT is a essential enzyme in the extracellular metabolism of plasma lipoproteins.

LCAT Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 441 amino acids (25-440) which includes a 25 amino acid His Tag fused at N-terminus and having a total molecular mass of 49.8 kDa.                   LCAT Human Recombinant is purified by proprietary chromatographic techniques.

UniProt Protein Function:LCAT: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD); also called Norum disease. LCATD is a disorder of lipoprotein metabolism characterized by inadequate esterification of plasmatic cholesterol. Two clinical forms are recognized: familial LCAT deficiency and fish-eye disease. Familial LCAT deficiency is associated with a complete absence of alpha and beta LCAT activities and results in esterification anomalies involving both HDL (alpha-LCAT activity) and LDL (beta-LCAT activity). It causes a typical triad of diffuse corneal opacities, target cell hemolytic anemia, and proteinuria with renal failure. Defects in LCAT are a cause of fish-eye disease (FED); also known as dyslipoproteinemic corneal dystrophy or alpha-LCAT deficiency. FED is due to a partial LCAT deficiency that affects only alpha-LCAT activity. It is characterized by low plasma HDL and corneal opacities due to accumulation of cholesterol deposits in the cornea ('fish-eye'). Belongs to the AB hydrolase superfamily. Lipase family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Lipid Metabolism - glycerophospholipid; EC 2.3.1.43; Transferase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular region; extracellular space

Molecular Function:apolipoprotein A-I binding; phosphatidylcholine-sterol O-acyltransferase activity; protein binding

Biological Process: cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; lipoprotein metabolic process; phosphatidylcholine biosynthetic process; phosphatidylcholine metabolic process; phospholipid metabolic process; reverse cholesterol transport

Disease: Fish-eye Disease; Lecithin:cholesterol Acyltransferase Deficiency

NCBI Summary:This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008]
UniProt Code:P04180
NCBI GenInfo Identifier:125993
NCBI Gene ID:3931
NCBI Accession:P04180.1
UniProt Secondary Accession:P04180,Q53XQ3,
UniProt Related Accession:P04180
Molecular Weight:49,578 Da
NCBI Full Name:Phosphatidylcholine-sterol acyltransferase
NCBI Synonym Full Names:lecithin-cholesterol acyltransferase
NCBI Official Symbol:LCAT  
NCBI Protein Information:phosphatidylcholine-sterol acyltransferase
UniProt Protein Name:Phosphatidylcholine-sterol acyltransferase
UniProt Synonym Protein Names:Lecithin-cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase
UniProt Gene Name:LCAT  
UniProt Entry Name:LCAT_HUMAN
View AllClose

Related Products