The Human FGF7 (KGF) ELISA Kit is specifically designed for the precise and accurate measurement of Fibroblast Growth Factor 7 (Keratinocyte Growth Factor) levels in human serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring dependable and replicable results for various research applications. Fibroblast Growth Factor 7, also known as Keratinocyte Growth Factor, is a key protein involved in cell growth, development, and tissue repair. It plays a crucial role in promoting the proliferation and differentiation of various cell types, making it an important molecule in the fields of regenerative medicine, wound healing, and cancer research.
By using the Human FGF7 (KGF) ELISA Kit, researchers can accurately quantify FGF7 levels in biological samples, allowing for in-depth investigation into its biological functions and potential therapeutic applications. This kit provides a valuable tool for studying the role of FGF7 in various physiological and pathological processes, ultimately advancing our understanding of this important growth factor.
Product Name:
Human FGF7/KGF ELISA Kit
SKU:
HUFI00118
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL (1ng=520mIU)
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
90-105%
89-98%
88-104%
EDTA Plasma (n = 5)
89-101%
88-100%
86-99%
Heparin Plasma (n = 5)
84-97%
84-100%
80-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-105
97
EDTA Plasma (n = 5)
85-103
93
Heparin Plasma (n = 5)
87-98
91
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
