FAM132B is a homeobox transcription factor which is implicated in numerous biological processes over the course of embryonic development, including neural crest cell differentiation, cardiac septation and sexual dimorphic brain development. FAM132B is expressed in the hypothalamus and anterior pituitary gland. The expression of FAM132B is regulated by genetic imprinting. FAM132B associated diseases include autism spectrum disorder, precocious puberty and intellectual disabilities.
Description
Human FAM132B ELISA
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Human FAM132B ELISA Kit |
| Product Code: | HUFI01392 |
| Size: | 96 Assays |
| Alias: | Erythroferrone, Protein FAM132B, Complement C1q tumor necrosis factor-related protein 15, Myonectin, C1QTNF15, CTRP15 |
| Detection Method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human FAM132B concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.094ng/ml |
| Range: | 0.156-10ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery | Matrices listed below were spiked with certain level of Human FAM132B and the recovery rates were calculated by comparing the measured value to the expected amount of Human FAM132B in samples.
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| Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human FAM132B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%) | Intra-Assay: CV<8% |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer | 5 |
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