Human ADAM10 (A Disintegrin And Metalloprotease 10) ELISA Kit
The Human ADAM10 (A Disintegrin and Metalloprotease 10) ELISA Kit is specifically designed for the accurate and precise quantification of ADAM10 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ADAM10 is a key enzyme involved in the proteolytic processing of proteins and is known to play a critical role in various cellular processes such as cell adhesion, migration, and signaling. Dysregulation of ADAM10 has been linked to numerous diseases including cancer, Alzheimer's disease, and inflammatory disorders, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development. By using the Human ADAM10 ELISA Kit, researchers can accurately measure ADAM10 levels in biological samples, providing valuable insights into the role of this enzyme in disease pathogenesis and progression. With its high level of accuracy and reproducibility, this kit is essential for advancing our understanding of ADAM10 biology and its implications in disease research.
Product Name:
Human ADAM10 (A Disintegrin And Metalloprotease 10) ELISA Kit
SKU:
HUES01500
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
