The HK1 Polyclonal Antibody (CAB0546) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using high-quality recombinant protein as an immunogen, is highly specific and sensitive for detecting HK1 in human samples. Validated for use in various applications, including Western blot and immunohistochemistry, this antibody allows for precise and accurate analysis of HK1 expression levels in different cell types.HK1, a hexokinase enzyme, is responsible for initiating the first step in glycolysis by catalyzing the phosphorylation of glucose to glucose-6-phosphate. Its role in energy metabolism and regulation makes it a crucial target for research in areas such as cancer, diabetes, and neurodegenerative diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HK1 Polyclonal Antibody
SKU:
CAB0546
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
Purification Method
Affinity purification
Gene ID
3098
RRID
AB_2757256
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using HK1 Rabbit pAb (CAB0546). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
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