The Histone H3 Antibody (CAB2352) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Hist3H3 protein, this antibody enables precise detection and analysis in various cell types, making it an ideal tool for studies in epigenetics and cancer research.Hist3H3 is a key player in the epigenetic regulation of gene expression, influencing important biological processes such as DNA replication, DNA repair, and transcriptional activation. Dysregulation of Hist3H3 has been implicated in various diseases including cancer, developmental disorders, and neurological conditions.
This antibody is validated for use in WB, IHC-P, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Histone H3 Antibody
SKU:
CAB2352
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP
5μg antibody for 5μg-10μg of Chromatin
Synonyms:
H3t, H3.4, H3/g, H3FT, H3C16, HIST3H3, Histone H3
Positive Sample:
HeLa, HepG2, Raji, Mouse liver, Rat lung
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
15kDa
Observed MW:
17kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2764312
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Histone H3 Rabbit pAb (CAB2352) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Histone H3 Rabbit pAb (CAB2352) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using Histone H3 Rabbit pAb (CAB2352) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation analysis of 150 μg extracts of MCF7 cells using 3 μg Histone H3 antibody (CAB2352). Western blot was performed from the immunoprecipitate using Histone H3 antibody (CAB2352) at a dilution of 1:500.
