The GUCY1A3 Antibody (CAB12710) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been extensively validated for use in various applications, including Western blot and immunofluorescence.GUCY1A3 plays a crucial role in mediating various physiological processes, including smooth muscle relaxation, neurotransmission, and vasodilation. Dysregulation of GUCY1A3 has been implicated in several diseases, such as cardiovascular disorders, erectile dysfunction, and neurological conditions. Therefore, studying the expression and activity of GUCY1A3 is essential for understanding its role in health and disease.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GUCY1A3 Antibody
SKU:
CAB12710
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
U-87MG, A-549, 293T, Mouse kidney, Mouse brain, Rat kidney, Rat spleen, Rat lung
Cellular Localization:
Cytoplasm.
Calculated MW:
77kDa
Observed MW:
77kDa
Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2982
RRID
AB_2759551
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GUCY1A3 Rabbit pAb (CAB12710) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunofluorescence analysis of L929 cells using GUCY1A3 Rabbit pAb (CAB12710) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
