The EGF Antibody (CAB2720) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. EGF is a crucial growth factor that plays a vital role in cell proliferation, survival, and migration. Dysregulation of EGF signaling has been implicated in various diseases, including cancer, where it promotes tumor growth and metastasis. By using the EGF Polyclonal Antibody, researchers can detect and quantify EGF expression in different cell types, providing insights into its roles in normal physiology and disease.
This antibody is validated for use in WB, ELISA, DB applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
EGF Antibody
SKU:
CAB2720
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
URG, HOMG4, EGF
Positive Sample:
Mouse kidney, 293T
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
134kDa
Observed MW:
160kDa
This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Purification Method
Affinity purification
Gene ID
1950
RRID
AB_2764571
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse kidney, using EGF Rabbit pAb (CAB2720) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from 293T cells, using EGF Rabbit pAb (CAB2720) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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