The E2F6 Antibody (CAB2718) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and is ideal for use in Western blot applications. By binding to the E2F6 protein, this antibody enables the detection and analysis of E2F6 expression in various cell types, making it a crucial component of studies in molecular biology and cancer research.E2F6 is known for its role in cell cycle control and its involvement in tumorigenesis, making it a promising target for cancer research.
This antibody is validated for use in WB, IF/ICC, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
E2F6 Antibody
SKU:
CAB2718
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP
5μg antibody for 10μg-15μg of Chromatin
Synonyms:
E2F-6, E2F6
Positive Sample:
Mouse lung
Cellular Localization:
Nucleus.
Calculated MW:
32kDa
Observed MW:
35kDa
This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1876
RRID
AB_2764570
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse lung using E2F6 Rabbit pAb (CAB2718) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of U2OS cells using E2F6 Rabbit pAb (CAB2718). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of 293T cells, using E2F6 antibody (CAB2718) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
