The [KO Validated] CIRBP Antibody (CAB6559) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in various research applications, including Western blotting.CIRBP, a stress-responsive protein, is known to play a critical role in regulating cellular processes such as RNA stabilization, gene expression, and cell survival under stressful conditions. Its involvement in processes related to inflammation, cell cycle regulation, and apoptosis makes it a target of interest in studies focused on cancer, neurological disorders, and metabolic diseases.
This antibody is validated for use in - applications and has demonstrated reactivity against - samples.
Western blot analysis of various lysates using [KO Validated] CIRBP Rabbit pAb (CAB6559) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and CIRBP knockout (KO) 293T cells, using [KO Validated] CIRBP Rabbit pAb (CAB6559) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using [KO Validated] CIRBP Rabbit pAb (CAB6559) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using [KO Validated] CIRBP Rabbit pAb (CAB6559) at dilution of 1:100 (20x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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