The Chicken CFD (Complement Factor D) ELISA Kit offered by AssayGenie is an advanced assay designed for the accurate measurement of complement factor D levels in chicken samples. This kit showcases exceptional sensitivity and specificity, guaranteeing precise and consistent results, thus making it a valuable tool for a variety of research investigations. Complement factor D is a key protein within the immune system, playing a critical role in the activation of the alternative complement pathway. Its functions are essential for the body's defense against infections and in maintaining immune homeostasis.
By measuring complement factor D levels, researchers can gain insights into immune responses and inflammatory processes in chickens, aiding in the study of infectious diseases, immune disorders, and vaccine development. With its robust performance and broad applicability, the Chicken CFD ELISA Kit from AssayGenie is a reliable choice for researchers seeking to unravel the complexities of the chicken immune system and advance scientific understanding in poultry health and disease.
Product Name:
Chicken CFD (Complement Factor D) ELISA Kit
SKU:
AEFI00027
Reactivity:
Chicken
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
93-100%
89-100%
80-98%
EDTA Plasma (n = 5)
86-105%
85-98%
86-98%
Heparin Plasma (n = 5)
85-104%
84-94%
82-97%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-104
96
EDTA Plasma (n = 5)
90-105
97
Heparin Plasma (n = 5)
85-100
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
