The CTSG Antibody (CAB5636) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to Cathepsin G protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component of immunology and cancer research studies.Cathepsin G is a serine protease that plays a crucial role in various physiological processes, including inflammation, tissue remodeling, and immune response modulation.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CTSG Antibody
SKU:
CAB5636
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CG, CATG, Cathepsin G
Positive Sample:
Raji, Mouse liver, Mouse spleen, Rat liver, Rat thymus
Cellular Localization:
Cell Surface.
Calculated MW:
29kDa
Observed MW:
29kDa
The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene.
Purification Method
Affinity purification
Gene ID
1511
RRID
AB_2766396
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Raji cells, using Cathepsin G Rabbit pAb (CAB5636) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of various lysates using Cathepsin G Rabbit pAb (CAB5636) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Cathepsin G Rabbit pAb (CAB5636) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using Cathepsin G Rabbit pAb (CAB5636) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
