The Canine TNF-alpha (Tumor Necrosis Factor Alpha) ELISA Kit is designed for the quantitative detection of Canine TNF-alpha (Tumor Necrosis Factor Alpha) levels in various biological samples. Tumor Necrosis Factor (TNF-α) is a potent pro-inflammatory cytokine produced primarily by macrophages, T lymphocytes, and natural killer cells in response to infection and tissue damage. It mediates a broad range of biological effects including apoptosis induction, NF-κB activation, and orchestration of systemic inflammatory responses. Elevated TNF-α is causally implicated in rheumatoid arthritis, inflammatory bowel disease, psoriasis, and septic shock, and TNF blockade represents one of the most successful therapeutic strategies in modern medicine. Assay Genie's Canine TNF-alpha (Tumor Necrosis Factor Alpha) ELISA Kit offers exceptional sensitivity and specificity with a sensitivity of 9.38 pg/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Canine TNF-alpha (Tumor Necrosis Factor Alpha) ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
