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Description
Apoptosis - Intrinsic (Mitochondrial) Antibody Sampler Pack
The Apoptosis - Intrinsic (Mitochondrial) Antibody Sampler Pack from Assay Genie brings together 8 rigorously validated antibodies spanning the key nodes of this pathway, supplied in a convenient 20µL trial size. It lets researchers screen an entire signalling axis in a single, cost-effective pack — validated for applications including ELISA, IF/ICC, IHC-P, IP, WB — before committing to full-size vials. Each antibody is also available individually in a full 100µL size.
| Target | Antibody | Cat. No. | Host | Reactivity | Applications | Size |
| Bcl-2 | Anti-Bcl-2 Polyclonal Antibody | CAB0208-20 | Rabbit | Human, Mouse, Rat | WBIHC-PIF/ICCIPELISA | 20µL |
| Bax | Anti-Bax [ARC5006-10] Monoclonal Antibody -Knockout Validated | CAB20227-20 | Rabbit | Human, Mouse, Rat | WBIF/ICCIPELISA | 20µL |
| Bak | Anti-Bak Antibody | CAB0204-20 | Rabbit | Human, Mouse, Rat | WBIPIF/ICCELISA | 20µL |
| Bcl-xL | Anti-Bcl-XL Polyclonal Antibody -Knockout Validated | CAB22241-20 | Rabbit | Human, Mouse, Rat | WBELISA | 20µL |
| Bad | Anti-Bad Monoclonal Antibody | CAB19595-20 | Rabbit | Human, Mouse, Rat | WBIHC-PELISA | 20µL |
| Cytochrome c | Anti-Cytochrome C Monoclonal Antibody -Knockout Validated | CAB4912-20 | Rabbit | Human, Mouse, Rat | WBIHC-PELISA | 20µL |
| Caspase-9 | Anti-Caspase-9 Antibody -Knockout Validated | CAB0281-20 | Rabbit | Human, Mouse, Rat | WBIHC-PIF/ICCELISA | 20µL |
| Cleaved Caspase-3 | Anti-Caspase-3 p12 [ARC0143] Monoclonal Antibody | CAB19664-20 | Rabbit | Human, Mouse, Rat | WBIHC-PIF/ICCELISA | 20µL |
The intrinsic, or mitochondrial, apoptotic pathway integrates intracellular stress signals such as DNA damage, growth factor withdrawal, and oncogenic stress into a committed decision to die. The pathway is governed by the BCL-2 family of proteins, whose opposing pro-survival and pro-death members set the threshold for mitochondrial outer membrane permeabilization. Anti-apoptotic guardians including Bcl-2 and Bcl-xL sequester the pro-apoptotic effectors and restrain cell death, while the multidomain effectors Bax and Bak oligomerize within the outer mitochondrial membrane to form pores once their inhibition is relieved. The BH3-only protein Bad acts as a sensitizer; when dephosphorylated it displaces effectors from Bcl-2 and Bcl-xL, tipping the balance toward permeabilization. Once Bax and Bak generate pores, Cytochrome c is released from the intermembrane space into the cytosol, where it nucleates assembly of the apoptosome together with Apaf-1 and dATP. This platform recruits and activates the initiator protease Caspase-9, which in turn cleaves and activates downstream executioner caspases. The appearance of Cleaved Caspase-3 marks irreversible commitment to apoptosis, driving the proteolysis of structural and regulatory substrates that produce the morphological hallmarks of programmed cell death. In cancer, this axis is frequently subverted: overexpression of Bcl-2 or Bcl-xL, loss of pro-apoptotic effectors, and attenuated Cytochrome c release allow malignant cells to evade death and resist chemotherapy and radiation. These dependencies have become therapeutic targets, exemplified by BH3-mimetic drugs that neutralize Bcl-2, restoring effector function and apoptotic signaling. Quantifying the relative abundance, phosphorylation state, and processing of these regulators provides a mechanistic readout of how close a cell sits to its apoptotic threshold. This pack brings together validated antibodies against Bcl-2, Bax, Bak, Bcl-xL, Bad, Cytochrome c, Caspase-9, and Cleaved Caspase-3 for studying the intrinsic mitochondrial apoptosis pathway.
| Analyte | Alternate Names |
| Bcl-2 | BCL2, B-cell lymphoma 2 |
| Bax | BAX, Bcl-2 associated X protein, BCL2L4 |
| Bak | BAK1, Bcl-2 homologous antagonist/killer, BAK, CDN1 |
| Bcl-xL | BCL2L1, Bcl-X, Bcl-XL |
| Bad | BAD, Bcl2 associated agonist of cell death, BBC2, BCL2L8 |
| Cytochrome c | CYCS, Cytochrome c somatic, HCS |
| Caspase-9 | CASP9, ICE-LAP6, MCH6, APAF3 |
| Cleaved Caspase-3 | CASP3, Apopain, CPP32, Yama (cleaved/active form) |
| Specification | Detail |
| Pack Format | 8 antibodies, 20µL each (trial size) |
| Storage | Store at -20°C upon receipt |
| Shelf Life | 12 months from date of receipt |
| Shipping | Gel pack |
| Scale-up | Every antibody is also available individually in a full 100µL size |
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