Nitrotyrosine Antibody [39B6] is a mouse monoclonal antibody directed against Nitrotyrosine, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF, IP, ELISA, FCM and AM. Reactivity is species independent. Supplied as IgG2a, purified by Protein G.
Nitrotyrosine is a post-translational modification formed by the nitration of tyrosine residues, often mediated by reactive nitrogen species such as peroxynitrite. It serves as a biomarker of nitrosative stress and is increasingly recognized for its role in neurodegenerative disease pathology. Nitrotyrosine-modified proteins are frequently detected in Alzheimer’s disease, Parkinson’s disease, and ALS, where they correlate with inflammation-induced tissue injury. Enzymes such as myeloperoxidase, cytochrome P450s, and superoxide dismutase catalyze tyrosine nitration, disrupting protein function and contributing to neuronal damage. As a marker of oxidative and nitrosative stress, nitrotyrosine provides mechanistic insight into redox imbalance and may serve as a diagnostic and therapeutic target in neurodegeneration. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, IHC, ICC/IF, IP, ELISA, FCM, AM
Antibody Isotype:
IgG2a
Purification:
Protein G Purified
Concentration:
1 mg/ml
Reactivity:
Species Independent
Recommended Dilution:
WB (1:1400), IHC (1:100); optimal dilutions for assays should be determined by the user.
Specificity:
Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
Guarantee:
12 months from date of dispatch
Immunogen:
3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
Alternative Names:
Nitrotyrosine, Nitro tyrosine, 3-Nitrotyrosine
References:
1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387. 2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8. 3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30. 4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45. 5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199. 6. Khan J. et al. (1998) Biochem J. 330(2): 795-801.
