Anti-Mouse ICAM-1 (CD54) [BE29G1] In Vivo Antibody - Low Endotoxin

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SKU:
IVMB0168
Product Type:
In Vivo Monoclonal Antibody
Clone:
BE29G1
Protein:
Inter-Cellular Adhesion Molecule 1
Isotype:
Rat IgG2a
Reactivity:
Mouse
Synonyms:
BB2
Synonyms:
CD54
Synonyms:
P3.58
Research Area:
Adhesion & Migration
Endotoxin Level:
Low Endotoxin
Host Species:
Rat
Applications:
Blocking
Applications:
IP
Applications:
WB
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Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse ICAM-1 (CD54) [BE29G1] In Vivo Antibody - Low Endotoxin
Product Code:IVMB0168
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:BE29G1
Protein:Inter-Cellular Adhesion Molecule 1
Product Type:Monoclonal Antibody
Synonyms:BB2, CD54, P3.58
Isotype:Rat IgG2a
Reactivity:Mouse
Immunogen:Purified Recombinant Mouse ICAM-1 (>98%)
Applications:B, IP, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0167Anti-Mouse ICAM-1 (CD54) [BE29G1] In Vivo Antibody - Ultra Low EndotoxinUltra Low
IVMB0168Anti-Mouse ICAM-1 (CD54) [BE29G1] In Vivo Antibody - Low EndotoxinLow
Applications:B, IP, WB
Reactivity:Mouse
Host Species:Rat
Specificity:Rat Anti-Mouse CD54 (Clone BE29G1) recognizes the (Mr 95 kD) cell surface glycoprotein known as intracellular adhesion molecule ICAM-1. ICAM-1 is the receptor for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18).1,2,3
Antigen Distribution:The CD54 antigen is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. It has been demonstrated that ICAM-1 enhances T-cell activation.
Immunogen:Purified Recombinant Mouse ICAM-1 (>98%)
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein1 and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines.2 ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.3

Technical Datasheet:View
Protein:Inter-Cellular Adhesion Molecule 1
Ligand/Receptor:CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Research Area:Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cell
Product Thumbnail
Rat IgG2a Isotype Control
Clone 1-1
Isotype Rat IgG2a
Endotoxin Level Low Endotoxin
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