Anti-Mouse H-2Kd (MHC Class I) In Vivo Antibody - Low Endotoxin

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SKU:
IVMB0178
Product Type:
In Vivo Monoclonal Antibody
Clone:
A4C8.1-Do9
Protein:
MHC Class I
Isotype:
Mouse IgG1 kappa
Reactivity:
Mouse
Synonyms:
Class I major histocompatibility antigen H-2Kd
Research Area:
MHC & Immune Cell-Specific
Endotoxin Level:
Low Endotoxin
Host Species:
Mouse
Applications:
FC
Applications:
WB
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Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse H-2Kd (MHC Class I) In Vivo Antibody - Low Endotoxin
Product Code:IVMB0178
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:A4C8.1-Do9
Protein:MHC Class I
Product Type:Monoclonal Antibody
Synonyms:Class I major histocompatibility antigen H-2Kd
Isotype:Mouse IgG1 κ
Reactivity:Mouse
Applications:FC, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:FC, WB
Recommended Usage:FC
The suggested concentration for clone A4C8.1-Do9 antibody for staining cells in flow cytometry is ≤ .25 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:Mouse
Host Species:Mouse
Specificity:Clone A4C8.1-Do9 recognizes an epitope on mouse MHC class I H-2K haplotype d.
Antigen Distribution:H-2Kd is ubiquitously expressed on nucleated cells from mice of the H-2Kd haplotype, including BALB/c.
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

H-2Kd antibody, clone A4C8.1-Do9, recognizes the major histocompatibility complex (MHC) class I H-2K haplotype d (H-2Kd). MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2Kd plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.

Technical Datasheet:View
Protein:MHC Class I
Research Area:Immunology, Innate Immunity
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