Introducing the Mouse Anti-PD-1 (CD279) [RMP1-14] Recombinant Monoclonal Antibody from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the PD-1 protein, a key immune checkpoint, making it ideal for research in immunology, particularly in studies related to immune regulation and cancer immunotherapy.
With a mouse IgG1 isotype, it ensures high purity and low endotoxin levels, perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. PD-1 is a critical protein expressed on the surface of T-cells, B-cells, and myeloid cells. It plays a significant role in the immune system by inhibiting immune responses, thus maintaining self-tolerance and preventing autoimmunity. This antibody allows researchers to study the mechanisms of immune regulation and develop therapeutic strategies for diseases such as cancer and autoimmune disorders.
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<1.0 EU/µg
Purity:
≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
B, FA, IHC, In Vivo, WB
Reactivity:
Mouse
Host Species:
Mouse-Rat Chimeric
Specificity:
Anti-PD1 antibody (clone RMP1-14) recognizes an epitope on mouse PD-1.
Antigen Distribution:
PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells.
Immunogen:
Mouse PD-1 transfected BHK cells
Concentration:
≤1.0 EU/mg
Endotoxin Level:
<1.0 EU/µg
Formulation:
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
This anti-PD1 antibody is a recombinant chimeric version of the original clone RMP1-14 antibody. The variable domain sequences are identical to the original clone RMP1-14; however, the constant region sequences have been switched from rat IgG2a to mouse IgG2a.
PD-1 is a 50-55 kD member of the B7 Ig superfamily. PD-1 is also a member of the extended CD28/CTLA-4 family of T cell regulators and is suspected to play a role in lymphocyte clonal selection and peripheral tolerance. The ligands of PD-1 are PD-L1 and PD-L2, and are also members of the B7 Ig superfamily. PD-1 and its ligands negatively regulate immune responses. PD-L1, or B7-Homolog 1, is a 40 kD type I transmembrane protein that has been reported to costimulate T cell growth and cytokine production. The interaction of PD-1 with its ligand PD-L1 is critical in the inhibition of T cell responses that include T cell proliferation and cytokine production. PD-L1 has increased expression in several cancers. Inhibition of the interaction between PD-1 and PD-L1 can serve as an immune checkpoint blockade by improving T-cell responses In vitro and mediating preclinical antitumor activity. Within the field of checkpoint inhibition, combination therapy using anti-PD1 in conjunction with anti-CTLA4 has significant therapeutic potential for tumor treatments. PD-L2 is a 25 kD type I transmembrane ligand of PD-1. Via PD-1, PD-L2 can serve as a co-inhibitor of T cell functions. Regulation of T cell responses, including enhanced T cell proliferation and cytokine production, can result from mAbs that block the PD-L2 and PD-1 interaction.
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's recombinant antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
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