The Legumain (LGMN) Monoclonal Antibody (CAB6829) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for use in Western blot applications. By binding to the Legumain protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in biochemistry, cell biology, and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Legumain (LGMN) Monoclonal Antibody
SKU:
CAB6829
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1864
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AEP, LGMN1, PRSC1, Legumain (LGMN)
Positive Sample:
Mouse brain, Mouse spleen, Mouse kidney, Rat kidney, Hep G2
Cellular Localization:
Lysosome.
Calculated MW:
49kDa
Observed MW:
36kDa/56kDa
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.
Purification Method
Affinity purification
Gene ID
5641
RRID
AB_2863540
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Legumain (LGMN) Rabbit mAb (CAB6829) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from Hep G2 cells using Legumain (LGMN) Rabbit mAb (CAB6829) at 1:4000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
