Anti-Human CD4 [OKT-4] In Vivo Antibody - Low Endotoxin

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SKU:
IVMB0012
Product Type:
In Vivo Monoclonal Antibody
Clone:
OKT-4
Protein:
CD4
Isotype:
Mouse IgG2b kappa
Reactivity:
Human
Synonyms:
CD4
Synonyms:
T4
Synonyms:
CD4 antigen (p55)
Synonyms:
CD4 antigen p55
Synonyms:
CD4 receptor
Synonyms:
T-cell surface antigen T4/Leu-3
Synonyms:
T-cell surface glycoprotein CD4
Endotoxin Level:
Low Endotoxin
Host Species:
Mouse
Applications:
CODEX
Applications:
ELISA
Applications:
FC
Applications:
ICC
Applications:
IF Staining
Applications:
IHC FF
Applications:
IHC FFPE
Applications:
IP
Applications:
Neutralizing
Applications:
WB
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Description

system_update_altTechnical Datasheet
Product Name:Anti-Human CD4 [OKT-4] In Vivo Antibody - Low Endotoxin
Product Code:IVMB0012
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:OKT-4
Protein:CD4
Product Type:Monoclonal Antibody
Synonyms:CD4, T4, CD4 antigen (p55), CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Isotype:Mouse IgG2b κ
Reactivity:Human
Immunogen:Human peripheral T cells
Applications:ELISA, FC, ICC, IF Staining, IHC FF, IHC FFPE, IP, N, PhenoCycler®, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:CODEX®, ELISA, FC, ICC, IF Staining, IHC FF, IHC FFPE, IP, N, WB
Recommended Usage:ELISA
FC
WB
Reactivity:Human
Host Species:Mouse
Specificity:Clone OKT4 recognizes Human CD4. This clone recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not interfere with each other.
Antigen Distribution:CD4 is expressed on most thymocytes, a subset of T cells, and monocytes/macrophages, and may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues.
Immunogen:Human peripheral T cells
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD4 is a 55 kD single-chain type I transmembrane glycoprotein and a member of the Ig superfamily. It is involved in the recognition of MHC class II molecules. Furthermore, CD4 is the primary receptor for HIV. CD4 initiates the early phase of T-cell activation via its association with the T-cell receptor complex and protein tyrosine kinase, Lck. In addition, CD4 functions as a mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system.

Technical Datasheet:View
Protein:CD4
Research Area:Immunology
Product Thumbnail
Mouse IgG2b Isotype Control
Clone MPC-11
Isotype Mouse IgG2b kappa
Endotoxin Level Low Endotoxin
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