The GNE Monoclonal Antibody (CAB20978) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for GNE and has been validated for use in various applications, including Western blot and immunohistochemistry.GNE, also known as UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, plays a crucial role in the production of sialic acids, which are important for cell-cell interactions, immune response, and development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
GNE Monoclonal Antibody
SKU:
CAB20978
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC2970
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NM, DMRV, IBM2, Uae1, GLCNE, GNE
Positive Sample:
Hep G2, Jurkat, K-562, Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
79kDa
Observed MW:
75kDa
The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
10020
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GNE Rabbit mAb (CAB20978) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
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