Cystatin B Monoclonal Antibody (CAB20886)

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SKU:
CAB20886
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
PME, ULD, CST6, EPM1, STFB, CPI-B, EPM1A, Cystatin B

Description

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Cystatin B Monoclonal Antibody (CAB20886)

The Cystatin B Monoclonal Antibody (CAB20886) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in various applications such as Western blotting and immunofluorescence.Cystatin B is a member of the cystatin family of protease inhibitors and is involved in inhibiting lysosomal cathepsins, enzymes that break down proteins. Dysregulation of Cystatin B has been implicated in various diseases, including neurodegenerative disorders and cancer.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Cystatin B Monoclonal Antibody
SKU:CAB20886
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC2833
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MMCG APSA TQPA TAET QHIA DQVR SQLE EKEN KKFP VFKA VSFK SQVV AGTN YFIK VHVG DEDF VHLR VFQS LPHE NKPL TLSN YQTN KAKH DELT YF
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PME, ULD, CST6, EPM1, STFB, CPI-B, EPM1A, Cystatin B
Positive Sample:U-87MG, HL-60 (Low expression control), Mouse liver, Mouse kidney, Rat kidney
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:11kDa
Observed MW:13kDa

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

Purification MethodAffinity purification
Gene ID1476
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Cystatin B Rabbit mAb (CAB20886) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of various lysates using Cystatin B Rabbit mAb (CAB20886) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.

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