The Calmegin Monoclonal Antibody (CAB19643) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody is raised in rabbits and has high reactivity with human samples. It is validated for use in Western blot applications, specifically binding to the Calmegin protein for detection and analysis in various cell types.Calmegin, a chaperone protein located in the endoplasmic reticulum, plays a crucial role in protein folding and quality control mechanisms within the cell. Dysregulation of Calmegin has been implicated in various diseases, including neurodegenerative disorders and cancer.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Calmegin Monoclonal Antibody
SKU:
CAB19643
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2213
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
PC-3, Mouse testis, Rat testis
Cellular Localization:
Endoplasmic Reticulum.
Calculated MW:
70kDa
Observed MW:
93kDa
Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility.
Purification Method
Affinity purification
Gene ID
1047
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Calmegin Rabbit mAb (CAB19643) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from Rat testis, using Calmegin Rabbit mAb (CAB19643) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of paraffin-embedded mouse testis using Calmegin Rabbit mAb (CAB19643) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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