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Description
AMPK Energy Sensing Antibody Sampler Pack
The AMPK Energy Sensing Antibody Sampler Pack from Assay Genie brings together 8 rigorously validated antibodies spanning the key nodes of this pathway, supplied in a convenient 20µL trial size. It lets researchers screen an entire signalling axis in a single, cost-effective pack — validated for applications including ELISA, IF/ICC, IHC-P, WB — before committing to full-size vials. Each antibody is also available individually in a full 100µL size.
| Target | Antibody | Cat. No. | Host | Reactivity | Applications | Size |
| AMPK-alpha | Anti-AMPKalpha2 Antibody -Knockout Validated | CAB7339-20 | Rabbit | Human, Mouse, Rat | WBIF/ICCELISA | 20µL |
| Phospho-AMPK-alpha | Anti-Phospho-AMPKalpha1-T183+AMPKalpha2-T172 [ARC62278] Monoclonal Antibody | CABP1441-20 | Rabbit | Human, Mouse, Rat | WBELISA | 20µL |
| LKB1/STK11 | Anti-LKB1 Antibody -Knockout Validated | CAB2122-20 | Rabbit | Human, Mouse, Rat | WBIHC-PIF/ICCELISA | 20µL |
| ACC | Anti-Acetyl-CoA Carboxylase Polyclonal Antibody | CAB23986-20 | Rabbit | Human Mouse Rat | WBELISA | 20µL |
| Phospho-ULK1-S555 | Anti-Phospho-ULK1-S555 Antibody | CABP0760-20 | Rabbit | Human, Mouse | WBELISA | 20µL |
| TSC2 | Anti-TSC2 Antibody | CAB0492-20 | Rabbit | Human, Mouse, Rat | WBIHC-PIF/ICCELISA | 20µL |
| Raptor | Anti-Raptor [ARC1375] Monoclonal Antibody | CAB8992-20 | Rabbit | Human, Mouse | WBIHC-PIF/ICCELISA | 20µL |
| ULK1 | Anti-ULK1 Antibody | CAB8529-20 | Rabbit | Human, Mouse, Rat | WBIF/ICCELISA | 20µL |
AMP-activated protein kinase (AMPK) is the central energy sensor of eukaryotic cells, a heterotrimeric serine/threonine kinase that monitors the cellular AMP:ATP and ADP:ATP ratios to restore energy homeostasis. When energy charge falls, adenine nucleotides bind the regulatory gamma subunit and promote phosphorylation of a conserved threonine in the activation loop of the catalytic AMPK-alpha subunit. The principal upstream kinase responsible for this modification is the tumor suppressor LKB1/STK11, which acts in complex with STRAD and MO25 to constitutively prime AMPK for activation under stress. The resulting Phospho-AMPK-alpha species marks the active enzyme and is a widely used readout of metabolic stress. Once engaged, AMPK switches off anabolic, ATP-consuming pathways and switches on catabolic, ATP-generating ones. It directly phosphorylates and inhibits acetyl-CoA carboxylase (ACC), the committed step of fatty acid synthesis, thereby shifting cells toward fatty acid oxidation. AMPK also restrains the mechanistic target of rapamycin complex 1 by phosphorylating TSC2, enhancing its GAP activity toward Rheb, and by directly phosphorylating Raptor, a core mTORC1 scaffold, which suppresses protein synthesis and growth. In parallel, AMPK promotes autophagy through activating phosphorylation of ULK1, including the characteristic Phospho-ULK1-S555 mark, coupling nutrient scarcity to the recycling of cellular components. Because this network governs the balance between growth and survival, its dysregulation contributes to type 2 diabetes, obesity, cardiovascular disease, and cancer, and AMPK is the molecular target of the widely prescribed antidiabetic agent metformin. Studying LKB1, AMPK-alpha and its phosphorylation, ACC, TSC2, Raptor, and the ULK1 axis together provides a comprehensive view of how cells perceive and respond to energetic stress. This pack brings together validated antibodies against these key targets to support robust investigation of AMPK-dependent energy sensing.
| Analyte | Alternate Names |
| AMPK-alpha | PRKAA1, PRKAA2, AMPK alpha-1/alpha-2 |
| Phospho-AMPK-alpha | PRKAA1, PRKAA2, AMPK alpha (phospho-Thr172) |
| LKB1/STK11 | STK11, LKB1, Serine/threonine kinase 11, PJS |
| ACC | ACACA, Acetyl-CoA carboxylase alpha, ACAC |
| Phospho-ULK1-S555 | ULK1, ATG1, Unc-51 like autophagy activating kinase 1 (phospho-Ser555) |
| TSC2 | Tuberin, Tuberous sclerosis 2, TSC4 |
| Raptor | RPTOR, Regulatory-associated protein of mTOR, KOG1 |
| ULK1 | Unc-51 like autophagy activating kinase 1, ATG1, ATG1A |
| Specification | Detail |
| Pack Format | 8 antibodies, 20µL each (trial size) |
| Storage | Store at -20°C upon receipt |
| Shelf Life | 12 months from date of receipt |
| Shipping | Gel pack |
| Scale-up | Every antibody is also available individually in a full 100µL size |
