The ALDH4A1 Antibody (CAB2595) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications. By specifically binding to the ALDH4A1 protein, researchers can accurately detect and analyze this enzyme in various cell types.ALDH4A1 is essential for converting pyrroline-5-carboxylate into ornithine, a process critical for cellular energy production and the maintenance of amino acid balance.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ALDH4A1 Antibody
SKU:
CAB2595
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P5CD, ALDH4, P5CDh, ALDH4A1
Positive Sample:
HepG2, Mouse kidney, Mouse liver, Rat kidney, Rat liver
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
62kDa
Observed MW:
62kDa
This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Purification Method
Affinity purification
Gene ID
8659
RRID
AB_2764480
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ALDH4A1 Rabbit pAb (CAB2595) at 1:6000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of A549 cells using ALDH4A1 Rabbit pAb (CAB2595).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
