The AGMAT Antibody (CAB12192) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been optimized for use in Western blot applications and is highly specific to human samples.Agmat is known for its involvement in the conversion of agmatine to putrescine, a process that is essential for the regulation of nitric oxide synthase activity and the production of polyamines. Dysregulation of Agmat has been linked to various diseases, including neurodegenerative disorders and cancer.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
AGMAT Antibody
SKU:
CAB12192
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AGMAT
Positive Sample:
HepG2, Rat kidney
Cellular Localization:
Mitochondrion.
Calculated MW:
38kDa
Observed MW:
35kDa
Predicted to enable agmatinase activity. Predicted to be involved in putrescine biosynthetic process from arginine, using agmatinase. Predicted to be located in mitochondrion.
Purification Method
Affinity purification
Gene ID
79814
RRID
AB_2759074
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using AGMAT Rabbit pAb (CAB12192) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
