Affinity Gel-conjugated Mouse anti DDDDK-Tag mAb (CABE061)
The Affinity Gel-conjugated Mouse anti DDDDK-Tag mAb (CABE061) is a high-quality antibody developed for reliable detection and analysis of target proteins. The FLAG-tag (DYKDDDDK) is used for protein detection, affinity chromatography purification, and the isolation of multi-subunit protein complexes under mild conditions that preserve complex integrity. Anti-FLAG antibodies enable highly specific detection across western blotting, immunofluorescence, co-immunoprecipitation, and structural biology applications.
This antibody is validated for use in IP applications and has demonstrated reactivity against Species independent samples.
Product Name:
Affinity Gel-conjugated Mouse anti DDDDK-Tag mAb
SKU:
CABE061
Size:
20μL, 50μL, 100μL, 200μL
Reactivity:
Species independent
Clone Number:
AMC0382
Conjugate:
Affinity Gel
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
IP
Recommended Dilution:
Synonyms:
DDDDK, DDDDK tag, DDDDK-tag
Observed MW:
RefertoFigures
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
Purification Method
Affinity purification
RRID
AB_2863789
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with GSK3B expression vector containing a DDDDK-tag with 30uL Affinity Gel-conjugated Mouse anti DDDDK-Tag mAb {Anti-DDDDK Affinity Gel} (CABE061).Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag antibody at 1:10000 dilution.
Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with IRF1 expression vector containing a DDDDK-tag with 30uL Affinity Gel-conjugated Mouse anti DDDDK-Tag mAb antibody（CABE061).Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag antibody at 1:10000 dilution.
