The 8-epi-PGF2 alpha (8-epi-Prostaglandin F2 alpha) ELISA Kit is specially designed for the precise measurement of 8-epi-PGF2 alpha levels in various biological samples including serum, plasma, and urine. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers working in the fields of oxidative stress, inflammation, and lipid peroxidation.8-epi-PGF2 alpha is a biomarker of oxidative stress and lipid peroxidation, playing a crucial role in the pathogenesis of various diseases such as cardiovascular disorders, asthma, and chronic kidney disease. By accurately quantifying 8-epi-PGF2 alpha levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the 8-epi-PGF2 alpha ELISA Kit is an essential tool for studying oxidative stress-related diseases and developing novel treatment strategies to improve patient outcomes.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
86-97
87-102
88-99
Average (%)
91
94
94
1:4
Range (%)
83-95
87-100
94-111
Average (%)
90
93
101
1:8
Range (%)
85-101
88-101
98-114
Average (%)
92
95
105
1:16
Range (%)
83-94
85-99
99-114
Average (%)
89
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
92-108
100
Cell Culture Media (n=8)
93-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.56
109.4
465.85
43.16
99.02
447.74
Standard deviation
1.85
5.48
31.96
3.06
7.65
34.79
C V (%)
4.07
5.01
6.86
7.09
7.73
7.77
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 50 μL
Specificity:
This kit recognizes Universal 8-epi-PGF2α in samples.No significant cross-reactivity or interference between Universal 8-epi-PGF2α and analogues was observed
