The YTHDF2 Polyclonal Antibody is a valuable tool for researchers studying the YTH domain family protein 2 (YTHDF2), a key player in mRNA metabolism and gene expression regulation. This antibody, derived from rabbits, exhibits high specificity for human samples and is validated for use in Western blotting applications. By binding to the YTHDF2 protein, this antibody enables accurate detection and analysis of YTHDF2 expression in various cell types, making it an ideal choice for studies in molecular biology and RNA biology.
YTHDF2 is known for its role in recognizing and promoting the degradation of m6A-modified mRNAs, thus influencing gene expression levels and cellular processes. Understanding the functions and mechanisms of YTHDF2 is crucial for unraveling its contributions to gene regulation, RNA metabolism, and various biological pathways. Research on YTHDF2 is particularly relevant in the fields of cancer biology, developmental biology, and RNA epigenetics, offering insights into potential therapeutic targets and disease mechanisms.
Product Name:
YTHDF2 Polyclonal Antibody
SKU:
CAB24287
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 143-243AA of human YTHDF2 (NP_001166599.1).
This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Purification Method:
Affinity purification
Gene ID:
51441
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using YTHDF2 Rabbit pAb (CAB24287) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
