TMP (Trimethoprim) ELISA Kit (FSES0068)

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SKU:

FSES0068

Product Type:

ELISA Kit

Applications:

ELISA

Research Area:

Food Science

Description
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Description

TMP (Trimethoprim) ELISA Kit

Kit Name:

TMP (Trimethoprim) ELISA Kit

Kit Size:

96 Assays

Test Principle:

This kit uses Competitive-ELISA as the method. It can detect Trimethoprim (TMP) in samples, such as raw milk, tissue, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, TMP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-TMP antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of TMP. The concentration of TMP in the samples can be calculated by comparing the OD of the samples to the standard curve.

Sensitivity:

0.15 ppb (ng/mL)

Reaction Mode:

25°C, 30min ˜15min

Detection Limit:

Tissue---1pp; Raw milk ---4ppb

Cross Reactivity:

TMP ---100%

Sample Recovery Rate:

90%±30%.

Kits Components:

Item	Specifications
ELISA Microtiter plate	96 wells
Standard Liquid	1.5mL each (0ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb, 12.15ppb)
HRP Conjugate	7mL
Antibody Working Solution	7mL
10×Concentrated Sample Solution	10mL
Substrate Reagent A	7mL
Substrate Reagent B	7mL
Stop Solution	7mL
20×Concentrated Wash Buffer	25mL
Plate Sealer	3 pieces
Sealed Bag	1 piece
Manual	1 copy

Instruments:

Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01g).

Micropipette:

Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).

Reagents:

Methanol, NaHCO3.

Storage And Expiry Date:

Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.