The TIFY10A Polyclonal Antibody (CAB21960) is a powerful tool for researchers studying TIFY10A, a transcription factor implicated in various biological processes, including growth and development, stress responses, and immune regulation. This antibody, produced in rabbits, exhibits high sensitivity and specificity for detecting TIFY10A in human samples, making it a valuable asset for Western blot applications. By targeting the TIFY10A protein, this antibody enables precise detection and analysis in a variety of cell types, offering researchers insight into the role of TIFY10A in immunology and cancer research.
TIFY10A, also known as a key regulatory protein, plays a crucial role in controlling gene expression and signaling pathways involved in immune responses and stress tolerance. Its involvement in these pathways makes TIFY10A a promising target for investigating diseases such as cancer, inflammatory disorders, and metabolic syndromes. By elucidating the function of TIFY10A, researchers can gain valuable insights that may lead to the development of novel therapeutic strategies aimed at targeting TIFY10A-mediated pathways for therapeutic intervention.
Product Name:
TIFY10A Polyclonal Antibody
SKU:
CAB21960
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Arabidopsis thaliana
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 154-253 of arabidopsis thaliana TIFY10A (NP_564075.1).
AtJAZ1; jasmonate-zim-domain protein 1; T29M8.5; T29M8_5; TIFY10A
Positive Sample:
Rosette leaf
Conjugate:
Unconjugated
Cellular Localization:
nucleus, plasma membrane
Calculated MW:
28kDa
Observed MW:
32kDa
JAZ1 is a nuclear-localized protein involved in jasmonate signaling. JAZ1 transcript levels rise in response to a jasmonate stimulus. JAZ1 can interact with the COI1 F-box subunit of an SCF E3 ubiquitin ligase in a yeast-two-hybrid assay only in the presence of jasmonate-isoleucine (JA-ILE) or coronatine. Application of jasmonate methyl ester to Arabidopsis roots reduces the levels of a JAZ1:GUS fusion protein, presumably by stimulating ubiquitin-proteasome-mediated degradation. The Jas domain appears to be important for JAZ1-COI1 interactions in the presence of coronatine. Two positive residues (R205 and R206) in the Jas domain shown to be important for coronatine -dependent COI1 binding are not required for binding AtMYC2.
Purification Method:
Affinity purification
Gene ID:
838501
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Rosette leaf, using TIFY10A Rabbit pAb (CAB21960) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
