The TAF12 Polyclonal Antibody is a high-quality research tool designed for studying TAF12, a key protein involved in transcriptional regulation and gene expression. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is suitable for use in Western blot experiments.TAF12 is a critical component of the TFIID transcription initiation complex, playing a crucial role in the activation of RNA polymerase II-dependent transcription. Its function in regulating gene expression makes it a valuable target for research in the fields of molecular biology and gene regulation.
By targeting the TAF12 protein, this antibody allows for the detection and analysis of TAF12 expression levels in various cell types, aiding in the understanding of its role in transcriptional regulation. This makes it an ideal tool for researchers studying gene expression, transcriptional control, and the mechanisms underlying various diseases and disorders related to aberrant gene regulation. Overall, the TAF12 Polyclonal Antibody offers a reliable and effective solution for investigating the function and regulation of TAF12 in a research setting.
Product Name:
TAF12 Polyclonal Antibody
SKU:
CAB21616
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human TAF12 (NP_001128690.1).
Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6883
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using TAF12 antibody (CAB21616) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 120s.
