The SETD3 Polyclonal Antibody is a crucial tool for researchers studying the SETD3 protein, an enzyme involved in the methylation of actin. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications.SETD3 is known for its role in regulating actin dynamics, which in turn influences cellular processes such as cell migration, division, and signaling. By targeting the SETD3 protein, researchers can gain valuable insights into the molecular mechanisms underlying these processes and their relevance in various biological pathways.
This antibody enables precise detection and analysis of SETD3 protein levels in different cell types, making it an essential tool for studies in cell biology, cytoskeletal dynamics, and developmental biology. Understanding the functions of SETD3 can lead to advancements in fields such as cancer research, neurobiology, and regenerative medicine.
Product Name:
SETD3 Polyclonal Antibody
SKU:
CAB21681
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 72-329 of human SETD3 (NP_115609.2).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
hSETD3; C14orf154; SETD3
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
67kDa
Observed MW:
67kDa
Enables protein-L-histidine N-tele-methyltransferase activity. Involved in actin modification and peptidyl-histidine methylation. Located in cytoplasm.
Purification Method:
Affinity purification
Gene ID:
84193
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using SETD3 Rabbit pAb (CAB21681) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 45s.
