The ROCK1 Polyclonal Antibody is a valuable tool for researchers studying the Rho-associated protein kinase 1 (ROCK1) pathway, which plays a key role in cellular functions such as cell migration, adhesion, and proliferation. This antibody, produced in rabbits, is highly specific and validated for use in Western blot applications with human samples.ROCK1 is a serine/threonine kinase that is known to be involved in various cellular processes, including actin cytoskeleton organization and cell shape changes. Dysregulation of the ROCK1 pathway has been implicated in diseases such as cancer, cardiovascular disorders, and neurodegenerative diseases, making it an important target for therapeutic intervention.
By using the ROCK1 Polyclonal Antibody, researchers can accurately detect and analyze the expression of ROCK1 in different cell types, providing valuable insights into the underlying mechanisms of various diseases. This antibody is an essential tool for studies in cell biology, cancer research, and drug development aimed at targeting the ROCK1 pathway.
Product Name:
ROCK1 Polyclonal Antibody
SKU:
CAB0144
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
Purification Method:
Affinity purification
Gene ID:
6093
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using ROCK1 Rabbit pAb (CAB0144) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.
![[KO Validated] ROCK1 Rabbit Polyclonal Antibody (CAB1008)](https://cdn11.bigcommerce.com/s-39x6lpnvxv/images/stencil/590x590/products/156938/155068/anti-rock1-antibody-cab1008ko-validated__09896__16651.1750770029.jpg?c=1 "[KO Validated] ROCK1 Rabbit Polyclonal Antibody (CAB1008)")
