Recombinant Human Pepsin A-4/PGA4 Protein (RPCB1195)
- SKU:
- RPCB1195
- Size:
- 10µg
- Tag:
- C-6His
- Reactivity:
- Human
- Expression Host:
- HEK293 cells
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Description
| Product Name: | Recombinant Human Pepsin A-4/PGA4 Protein |
| SKU: | RPCB1195 |
| Size: | 10µg |
| Tag: | C-6His |
| Reactivity: | Human |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Pepsin A-4/PGA4 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <0.001EU/μg |
| Purity: | > 97% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 643847 |
PGA4 (Pepsinogen 4, group I), or Pepsinogen A, is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen, pepsinogen, whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed, and does not digest the body's own proteins in the stomach's lining. Five types of zymogens of pepsins, gastric digestive proteinases, are known: pepsinogens A, B, and F, progastricsin, and prochymosin. There are two major groups of pepsinogen, namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin), and each frequently has isozymogens. The PGA3, PGA4 and PGA5 genes encode identical human pepsinogen A enzymes.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P0DJD7 |
 | Recombinant Human Pepsin A-4/PGA4 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45 kDa. |
 | Recombinant Human Pepsin A-4/PGA4 cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (RD,Catalog # ES002). The specific activity is >27408 pmol/min/μg, as measured under the described conditions. |
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