Description
| Product Name: | Recombinant Human NSE/ENO2 Protein |
| SKU: | RPCB1761 |
| Size: | 10µg |
| Tag: | N-His |
| Reactivity: | Human |
| Expression Host: | E. coli |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human NSE/ENO2 Protein , tested reactivity inE. coliand has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <1EU/μg |
| Purity: | > 80% as determined by by reducing SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of 20mM Tris-HCl, 100mM KCl, 5mM MgSO4, pH 7.5. |
| Gene ID: | 2026 |
Gamma-enolase, also known as Enolase 2, belongs to the enolase family. The alpha/alpha homodimer of ENO2 is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jakob disease. ENO2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds to cultured neocortical neurons and promotes cell survival in a calcium-dependent manner.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P09104 |
 | Recombinant Human NSE/ENO2 Protein was determined by SDS- PAGE with Coomassie Blue, showing a band at 55 kDa. |
