Recombinant Human IFN-alpha H2/IFNA14 Protein (RPCB1898)
- SKU:
- RPCB1898
- Size:
- 10µg
- Tag:
- C-His
- Reactivity:
- Human
- Expression Host:
- HEK293 cells
Description
| Product Name: | Recombinant Human IFN-alpha H2/IFNA14 Protein |
| SKU: | RPCB1898 |
| Size: | 10µg |
| Tag: | C-His |
| Reactivity: | Human |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human IFN-alpha H2/IFNA14 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <0.01EU/μg of the protein by LAL method. |
| Purity: | > 95% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 3448 |
Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors . Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology . Structurally, type I IFNs belong to the class of five helical_x001E_bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds. The extracellular domain (ECD) of mature human IFNA14, shares 58% aa sequence identity with mouse IFNA14. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha_x001E_subunit) and IFNAR2 (beta-subunit). Individual IFNA subtypes are known to display unique efficacies to viral protection, and IFNA14 has been shown to be a strong inducer of IFN-stimulated genes and anti-viral protection . IFNA14 has been shown to be potent against HIV-1 by up_x001E_regulating the transcription of two intrinsic restriction factors with well-established anti-HIV-1 activity, MX2 and tetherin.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P01570 |
 | Recombinant Human IFN-alpha H2/IFNA14 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-30 kDa. |
 | Recombinant Human IFN-alpha H2/IFNA14 was measured in a cell cytotoxicity assay using TF-1 cells. The ED₅₀ for this effect is 0.47_x0000_1.88 ng/mL, corresponding to a specific activity of 5.32×105~2.13×106 units/mg. |
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