Rat TFF3 Recombinant Protein (RPPB0966)
- SKU:
- RPPB0966
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Rat
- Target:
- TFF3
- Synonyms:
- Trefoil factor 3
- Intestinal trefoil factor
- rITF
- Polypeptide P1B
- Source:
- Escherichia Coli
- Uniprot:
- Q03191
Description
| Product Name: | Rat TFF3 Recombinant Protein |
| Product Code: | RPPB0966 |
| Size: | 10µg |
| Species: | Rat |
| Target: | TFF3 |
| Synonyms: | Trefoil factor 3, Intestinal trefoil factor, rITF, Polypeptide P1.B, rP1.B, Tff3, Itf. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | TFF3 protein filtered (0.4µm) and lyophilized in 0.5mg/ml in 20mM TRIS and 50mM NaCl, pH 7.5. |
| Solubility: | It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MQEFVGLSPS QCMVPANVRV DCGYPTVTSE QCNNRGCCFD SSIPNVPWCF KPLQETECT F DYKDDDDK |
Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, relatively through an epidermal growth factor receptor-dependent pathway. TFF3 overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 is normally expressed in hepatocellular carcinoma and its expression associates with tumor grade.
The Trefoil Factor-3 Rat was constructed as a recombinant protein with a 9 a.a C-terminal fusion of Flag-Tag (1 aa N-terminal+8 aa C-terminal). The TFF3 Rat produced in E.coli, is 7.7kDa protein containing a total of 68 amino acid residues.
| UniProt Protein Function: | Function: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen) |
| UniProt Protein Details: | By similarity. Subunit structure: Monomer By similarity. Homodimer; disulfide-linked By similarity. Subcellular location: Secreted › extracellular space › extracellular matrix › apical lamina Ref.1 Ref.7. Tissue specificity: Expressed in goblet cells of the intestines, and colon, in paraventricular hypothalamus and supraoptic nuclei. Weakly expressed in gastric epithelial cells (at protein level). Expressed by goblet cells of small and large intestinal epithelia, kidney and stomach. Expressed in the paraventricular hypothalamus, arcuate nucleus and amygdala of the brain. Weakly expressed in gastric epithelial cells. Ref.1 Ref.3 Ref.5 Ref.8 Ref.9 Induction: Up-regulated by hypoxia in gastric epithelial cells. Up-regulated by hypoxia-inducible factor 1 alpha (HIF1A). Ref.9 Sequence similarities: Contains 1 P-type (trefoil) domain. |
| NCBI Summary: | plays a role in epithelial wound healing; involved in maintenance of the intestinal mucosa [RGD, Feb 2006] |
| UniProt Code: | Q03191 |
| NCBI GenInfo Identifier: | 386869333 |
| NCBI Gene ID: | 25563 |
| NCBI Accession: | NP_037174.2 |
| UniProt Secondary Accession: | Q03191,Q68FZ2, |
| UniProt Related Accession: | Q03191 |
| Molecular Weight: | 33.6kDa |
| NCBI Full Name: | trefoil factor 3 |
| NCBI Synonym Full Names: | trefoil factor 3, intestinal |
| NCBI Official Symbol: | Tff3 |
| NCBI Official Synonym Symbols: | ITF |
| NCBI Protein Information: | trefoil factor 3; polypeptide P1.B; intestinal trefoil factor |
| UniProt Protein Name: | Trefoil factor 3 |
| UniProt Synonym Protein Names: | Intestinal trefoil factor; rITF; Polypeptide P1.B |
| Protein Family: | Trefoil factor |
| UniProt Gene Name: | Tff3 |
| UniProt Entry Name: | TFF3_RAT |
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