Description
| Product Name: | Rat Carboxypeptidase B Recombinant Protein |
| Product Code: | RPPB1468 |
| Size: | 15mg |
| Species: | Rat |
| Target: | Carboxypeptidase B |
| Synonyms: | Carboxypeptidase B, Cpb1, Cpb. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered lyophilized powder. |
| Formulation: | The protein was lyophilized with 100mM NaCl, mannitol and 20mM Tris pH-7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18M?-cm H2O or 25mM Tris-HCl pH 7.65 not less than 100�g/ml , which can then be further diluted to other aqueous solutions. |
| Stability: | Store the lyophilized Carboxypeptidase-B at 4°C. Upon reconstitute the protein should be stored at 4°C for 2 weeks and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Biological Activity: | 170 units/mg protein. |
Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 34.5 kDa, optimun pH-7.9, and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA, 1,10-phenanthroline.
Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 31kDa is purified by standard chromatography techniques. Recombinant Rat Carboxypeptidase-B is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carboxypeptidase-B is free from protease inhibitors such as PMSF and EDTA.
| NCBI Summary: | putative carboxypeptidase B protein [RGD, Feb 2006] |
| UniProt Code: | P19223 |
| NCBI GenInfo Identifier: | 115886 |
| NCBI Gene ID: | 24271 |
| NCBI Accession: | P19223.1 |
| UniProt Related Accession: | P19223 |
| Molecular Weight: | 47,515 Da |
| NCBI Full Name: | Carboxypeptidase B |
| NCBI Synonym Full Names: | carboxypeptidase B1 |
| NCBI Official Symbol: | Cpb1�� |
| NCBI Official Synonym Symbols: | Cpb�� |
| NCBI Protein Information: | carboxypeptidase B |
| UniProt Protein Name: | Carboxypeptidase B |
| UniProt Gene Name: | Cpb1�� |
