| Kit Name: | OTC (Oxytetracycline) ELISA Kit |
| Kit Size: | 96 Assays |
| Test Principle: | This kit uses Competitive-ELISA as the method. It can detect Tetracyclines (TCS) in samples, such as tissue, honey, etc. |
| Sensitivity: | 0.05 ppb (ng/mL) |
| Reaction Mode: | 37°C, 30 min˜30 min˜15 min |
| Detection Limit: | Tissue, Liver, Eggs---0.4 ppb; Honey---2 ppb; Urine---0.5 ppb. |
| Cross Reactivity: | Tetracycline ---100%, Oxytetracycline ---100%, Chlortetracycline---16.7%, Deoxytetracycline ---4.2%. |
| Sample Recovery Rate: | Tissue, Liver, Eggs---85%±20%, Honey---75%±20%, Urine---80%±20%. |
| Kits Components: | | Item | Specifications | | ELISA Microplate | 96 wells | | High Concentrated Standard (1.0 ppm) | 1 mL | | Standard (empty bottle) | (0 ppb,0.05 ppb,0.15 ppb, 0.45 ppb, 1.35 ppb, 4.05 ppb) | | HRP Conjugate | 11 mL | | Antibody Working Solution | 5.5 mL | | Substrate Reagent A | 6 mL | | Substrate Reagent B | 6 mL | | Stop Solution | 6 mL | | 20×Concentrated Wash Buffer | 40 mL | | 5×Reconstitution Buffer | 50 mL | | Plate Sealer | 3 pieces | | Sealed Bag | 1 piece | | Manual | 1 copy | |
| Other materials required but not supplied: | | Item | Specifications | | Instruments: | Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g). | | High-precision transferpettor: | Single-channel (20-200 µL, 100-1000 µL), Multi-channel (300 µL). | | Reagents: | N, N-Dimethylformamide (DMF). | |
| Storage And Expiry Date: | Store the kit at 2-8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2-8°C. |
