Mouse FLT1 D7 Recombinant Protein (RPPB5839)
- SKU:
- RPPB5839
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- FLT1 D7
- Synonyms:
- FLT1
- Tyrosine-protein kinase receptor FLT
- Source:
- Insect Cells
- Uniprot:
- P35969
Description
| Product Name: | Mouse FLT1 D7 Recombinant Protein |
| Product Code: | RPPB5839 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | FLT1 D7 |
| Synonyms: | FLT-1, FLT1, Tyrosine-protein kinase receptor FLT, Flt-1, Tyrosine-protein kinase FRT, Fms-like tyrosine kinase 1, VEGFR-1. |
| Source: | Insect Cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | FLT1 D1-7 was lyophilized from a concentrated (1 mg/ml) sterile solution containing PBS Buffer. |
| Solubility: | It is recommended to reconstitute the lyophilized FLT1 Fc/Chimera in PBS not less than 50µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized FLT-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FLT1 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | YGSGSKLKVP ELSLKGTQHV MQAGQTLFLK CRGEAAHSWS LPTTVSQEDK RLSITPPSAC GRDNRQFCST LTLDTAQANH TGLYTCRYLP TSTSKKKKAE SSIYIFVSDA GSPFIEMHTD IPKLVHMTEG RQLIIPCRVT SPNVTVTLKK FPFDTLTPDG QRITWDSRRG FIIANATYKE IGLLNCEATV NGHLYQTNYL THRQTNTILD VQIRPPSPVR LLHGQTLVLN CTATTELNTR VQMSWNYPGK ATKRASIRQR IDRSHSHNNV FHSVLKINNV ESRDKGLYTC RVKSGSSFQS FNTSVHVYEK GFISVKHRKQ PVQETTAGRR SYRLSMKVKA FPSPEIVWLK DGSPATLKSA RYLVHGYSLI IKDVTTEDAG DYTILLGIKQ SRLFKNLTAT LIVNVKPQIY EKSVSSLPSP PLYPLGSRQV LTCTVYGIPR PTITWLWHPC HHNHSKERYD FCTENEESFI LDPSSNLGNR IESISQRMTV IEGTNKTVST LVVADSQTPG IYSCRAFNKI GTVERNIKFY VTDVPNGFHV SLEKMPAEGE DLKLSCVVNK FLYRDITWIL LRTVNNRTMH HSISKQKMAT TQDYSITLNL VIKNVSLEDS GTYACRARNI YTGEDILRKT EVLVRDSEAP HLLQNLSDYE VSISGSTTLD CQARGVPAPQ ITWFKNNHKI QQEPGIILGP GNSTLFIERV TEEDEGVYRC RATNQKGAVE SAAYLTVQGT SDKSNAASDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPMLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK |
| Biological Activity: | The activity of sVEGFR-1/Fc was determined by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells. |
Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signalling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation. Differential splicing of the flt-1 gene leads to the formation of a secreted, soluble variant of VEGFR-1 (sVEGFR-1). No naturally occurring, secreted forms of VEGFR-2 have so far been reported. The binding of VEGF165 to VEGFR-2 is dependent on heparin.
Soluble FLT1 Mouse Recombinant fused with the Fc part of human IgG1 produced in baculovirus is disulfide-linked homodimeric , polypeptide containing 965 amino acids. The monomers have a molecular mass of 130 kDa. The soluble receptor protein contains all 7 extracellular domains (Tyr23-Asn757), which contain all the information necessary for high affinity ligand binding.
| UniProt Protein Function: | VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; VEGFR family Cellular Component: focal adhesion; membrane; integral to plasma membrane; integral to membrane; plasma membrane; receptor complex; endosome Molecular Function:transferase activity; identical protein binding; vascular endothelial growth factor receptor activity; growth factor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity Biological Process: cell migration; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; multicellular organismal development; positive regulation of phosphoinositide 3-kinase activity; chemotaxis; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; patterning of blood vessels; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; response to hypoxia; blood vessel morphogenesis; angiogenesis; sprouting angiogenesis; embryonic morphogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration |
| UniProt Code: | P35969 |
| NCBI GenInfo Identifier: | 549319 |
| NCBI Gene ID: | 14254 |
| NCBI Accession: | P35969.1 |
| UniProt Secondary Accession: | P35969,O55094, Q61517, |
| UniProt Related Accession: | P35969 |
| Molecular Weight: | 149,876 Da |
| NCBI Full Name: | Vascular endothelial growth factor receptor 1 |
| NCBI Synonym Full Names: | FMS-like tyrosine kinase 1 |
| NCBI Official Symbol: | Flt1 |
| NCBI Official Synonym Symbols: | Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757 |
| NCBI Protein Information: | vascular endothelial growth factor receptor 1; embryonic receptor kinase 2; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; vascular endothelial growth factor receptor-1 |
| UniProt Protein Name: | Vascular endothelial growth factor receptor 1 |
| UniProt Synonym Protein Names: | Embryonic receptor kinase 2; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase receptor FLT |
| Protein Family: | Vascular endothelial growth factor receptor |
| UniProt Gene Name: | Flt1 |
| UniProt Entry Name: | VGFR1_MOUSE |
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