Human Ubiquitin G76A Recombinant Protein (RPPB5065)
- SKU:
- RPPB5065
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- Ubiquitin G76A
- Synonyms:
- Ubiquitin
- Ribosomal Protein S27a
- CEP80
- UBA80
- Source:
- Escherichia Coli
- Uniprot:
- P62988
Description
| Product Name: | Human Ubiquitin G76A Recombinant Protein |
| Product Code: | RPPB5065 |
| Size: | 20µg |
| Species: | Human |
| Target: | Ubiquitin G76A |
| Synonyms: | Ubiquitin, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A, Ubiquitin G76A. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | Diluted in PBS plus 5% glycerol. |
| Stability: | Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
The conserved 76 amino acid protein ubiquitin (Ub) regulates a host of intracellular processes through its enzymatic conjugation to other cellular proteins.Ubiquitination occurs through sequential steps catalyzed by activating (E1), conjugating (E2), and ligase (E3) enzymes. The final step results in the formation of an isopeptide bond between Ub’s C-terminal glycine residue (G76) and a lysine residue of the target protein, although N-terminal ubiquitination is also known.Outcomes of this modification include destabilization of the conjugated protein, altered protein trafficking and functional modulation.After targeting the protein for specific localizations, ubiquitin is released from the substrate by deubiquitinating enzymes.A mutant ubiquitin, having a Gly to Ala substitution at the C-terminus (G76A ubiquitin) supported several downstream reactions of the proteolytic pathway but inhibits the deubiquitination process.As consequence, the Ub derivative becomes irreversibly conjugated to protein, shifting the equilibrium between the bound and unbound form in the direction of conjugation, at the expense of the free form.
Recombinant human ubiquitin featuring a Gly76 to Ala76 mutation that, by inhibiting the ubiquitin hydrolases, prevents the removal of ubiquitin from protein ubiquitin conjugates. Ubiquitin G76A is expressed in E.coliand purified by ion-exchange chromatography.
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62988 |
| NCBI GenInfo Identifier: | 51703339 |
| NCBI Gene ID: | 7311 |
| NCBI Accession: | P62988.1 |
| UniProt Secondary Accession: | P62988 |
| UniProt Related Accession: | P62988,P62987 |
| Molecular Weight: | |
| NCBI Full Name: | |
| NCBI Synonym Full Names: | ubiquitin A-52 residue ribosomal protein fusion product 1 |
| NCBI Official Symbol: | UBA52 |
| NCBI Official Synonym Symbols: | L40; CEP52; RPL40; HUBCEP52 |
| NCBI Protein Information: | ubiquitin-60S ribosomal protein L40 |
| UniProt Protein Name: | |
| UniProt Synonym Protein Names: | |
| Protein Family: | Ubiquitin |
| UniProt Gene Name: | |
| UniProt Entry Name: |
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