Human UBE2F Recombinant Protein (RPPB2375)
- SKU:
- RPPB2375
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- UBE2F
- Synonyms:
- Ubiquitin-conjugating enzyme E2F (putative)
- NEDD8 carrier protein UBE2F
- NEDD8 protein ligase UBE2F
- NEDD8-conjugating enzyme 2
- Source:
- Escherichia Coli
- Uniprot:
- Q969M7
Description
| Product Name: | Human UBE2F Recombinant Protein |
| Product Code: | RPPB2375 |
| Size: | 20µg |
| Species: | Human |
| Target: | UBE2F |
| Synonyms: | Ubiquitin-conjugating enzyme E2F (putative), NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, NEDD8-conjugating enzyme UBE2F, NCE2, EC 6.3.2.-. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The UBE2F solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 1mM DTT and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMLTLAS KLKRDDGLKG SRTAATASDS TRRVSVRDKL LVKEVAELEA NLPCTCKVHF PDPNKLHCFQ LTVTPDEGYY QGGKFQFETE VPDAYNMVPP KVKCLTKIWH PNITETGEIC LSLLREHSID GTGWAPTRTL KDVVWGLNSL FTDLLNFDDP LNIEAAEHHL RDKEDFRNKV DDYIKRYAR |
Ubiquitin-conjugating enzyme E2 F (UBE2F) is a member of the ubiquitin-conjugating enzyme family and UBE2F subfamily. Ubiquitin-conjugating enzymes or sometimes as ubiquitin-carrier enzymes, run the second step in the ubiquitination reaction which targets a protein for degradation using the proteasome. The UBE2F receives the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.RBX2-UBE2F complex neddylates specific target proteins, for example CUL5 as can be seen from the specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1.
UBE2F Human Recombinant produced in E. coli is a single polypeptide chain containing 209 amino acids (1-185) and having a molecular mass of 23.7 kDa.UBE2F is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UBE2F: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-; Ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 2q37.3 Cellular Component: cytoplasm Molecular Function:protein binding; ubiquitin protein ligase binding; ATP binding; NEDD8 ligase activity; ligase activity Biological Process: protein neddylation; protein ubiquitination |
| UniProt Code: | Q969M7 |
| NCBI GenInfo Identifier: | 74751725 |
| NCBI Gene ID: | 140739 |
| NCBI Accession: | Q969M7.1 |
| UniProt Related Accession: | Q969M7 |
| Molecular Weight: | Predicted Molecular Mass: 24.8kDaAccurate Molecular Mass: 25kDa |
| NCBI Full Name: | NEDD8-conjugating enzyme UBE2F |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 F (putative) |
| NCBI Official Symbol: | UBE2F |
| NCBI Official Synonym Symbols: | NCE2 |
| NCBI Protein Information: | NEDD8-conjugating enzyme UBE2F |
| UniProt Protein Name: | NEDD8-conjugating enzyme UBE2F |
| UniProt Synonym Protein Names: | NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; Ubiquitin-conjugating enzyme E2 F |
| Protein Family: | NEDD8-conjugating enzyme |
| UniProt Gene Name: | UBE2F |
| UniProt Entry Name: | UBE2F_HUMAN |
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